Studies on Rapid Identification of Phytopathogenic Microorganisms.

RIFLE: Rapid Identification of Microorganisms by Fragment Length Evaluation

Biological macromolecules represent a valuable source of information for the identification and phylogenetic classification of microorganisms. One of the most commonly used macromolecules for this task is the 16S rDNA. The WWW-based RIFLE system presented here supports large-scale identification tasks by comparing 16S rDNA restriction patterns to a database of restriction patterns derived from ...

Comparative Analyses of Exoproteinases Produced by Three Phytopathogenic Microorganisms

Proteinases secreted by the oomycete Phytophthora infestans (Mont.) de Bary, Rhizoctonia solani, and Fusarium culmorum belonging to different families of fungi have been studied to determine if the exoenzyme secretion depends on the environmental conditions and the phylogenetic position of the pathogen. The substrate specificity of the extracellular proteinases of F. culmorum, R. solani, and P....

Development of conductive polymer analysis for the rapid detection and identification of phytopathogenic microbes.

ABSTRACT Conductive polymer analysis, a type of electronic aroma detection technology, was evaluated for its efficacy in the detection, identification, and discrimination of plant-pathogenic microorganisms on standardized media and in diseased plant tissues. The method is based on the acquisition of a diagnostic electronic fingerprint derived from multisensor responses to distinct mixtures of v...

Studies on the Fine Structure of Microorganisms

The fine structure of cells of Saccharomyces cerevisiae engaged in the formation of ascospores was studied in electron micrographs of ultrathin sections. Although the mode of the first reduction division could not be clearly determined, the second nuclear division appeared to proceed in a manner similar to that observed previously during vegetative division. That is, division by constriction of...

Studies on Bacteriophage and Metabolic Identification of Brucella Strains